Inhibition of Electrochemically Controlled Bioluminescence of Bacterial Luciferase by n-Alkyl Alcohols
نویسندگان
چکیده
منابع مشابه
Inhibition of electrochemically controlled bioluminescence of bacterial luciferase by n-alkyl alcohols.
An electrochemical system has been developed in order to assay the effect of hydrophobic molecules on the bioluminescence of bacterial luciferase (BL). The inhibition of BL luminescence by the long-chain n-alkyl alcohol has been examined using this system. The 1-heptanol, 1-octanol, 1-nonanol, 1-decanol, 1-undecanol and 1-dodecanol inhibited the BL reaction in a dose-dependent manner. The IC(50...
متن کاملBioluminescence inhibition of bacterial luciferase by aliphatic alcohol, amine and carboxylic acid: inhibition potency and mechanism.
The inhibitory effects of hydrophobic molecules on the bacterial luciferase, BL, luminescence reaction were analyzed using an electrochemically-controlled BL luminescence system. The inhibition potency of alkyl amines, C(n)NH(2), and fatty acids, C(m)COOH (m = n - 1), on the BL reaction increased with an increase in the alkyl chain-length of these aliphatic compounds. C(m)COOH showed lower in...
متن کاملBacterial luciferase: bioluminescence emission using lumanzines as substrates
A C f E R I A L L U C I F E R A S E : B I O L U M I N E S C E N C E E M I S S I O N U S I N G L U M A Z I N E S A S S U B S T R A T E S P e t e r M a c h e r o u x a n d S a n d r o G h i s l a U n i v e r s i t y o f K o n s t a n z , F a c u l t y o f B i o l o g y , P . O . B o x 5 5 6 0 , D 7 8 4 3 4 K o n s t a n z , G e n n a n y J . W o o d l a n d H a s t i n g s H a r v a r d U n i v e...
متن کاملMeasurement of Michaelis Constants for ATP and Mg2+ in Bioluminescence Reaction of Luciferase by a Home-Made Luminometer
Effects of ATP and Mg2+ concentrations on bioluminescence reaction of luciferase (Photinus pyralis) were investigated by home-made luminometer. The Michaelis constants of the enzyme for ATP and Mg2+ obtained from the Lineweaver-Burk graph, were 61.9 mM ±3.3 mM and 251.6mM ± 39.0mM...
متن کاملInhibition of Firefly Luciferase by General Anesthetics: Effect on In Vitro and In Vivo Bioluminescence Imaging
UNLABELLED Bioluminescence imaging is routinely performed in anesthetized mice. Often isoflurane anesthesia is used because of its ease of use and fast induction/recovery. However, general anesthetics have been described as important inhibitors of the luciferase enzyme reaction. AIM To investigate frequently used mouse anesthetics for their direct effect on the luciferase reaction, both in vi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Sciences
سال: 2011
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.27.357